[Disclaimer: material deposited here is available without warranty, pulse sequence implementation may need to be adapted depending on your spectrometer]

 

Experiment

Pulse program / dataset

Explanation

Reference

BUSS decoupling

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BUSS decoupling shape

Decoupling shape for Bruker spectrometers, see supplement of Angew Chem paper for details

Schilling F, Warner LR, Gershenzon NI, Skinner TE, Sattler M, Glaser SJ.
Next-Generation Heteronuclear Decoupling for High-Field Biomolecular NMR Spectroscopy.
(2014) Angew Chem Int Ed Engl. 53, 4475–4479.
doi: 10.1002/anie.201400178 [Pubmed].

(lr) HNN-COSY

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J(N-N) correlation in isotope-labeled RNA

pulse program and Bruker AvanceIII dataset

Dallmann A, Simon B, Duszczyk MM, Kooshapur H, Pardi A, Bermel W, Sattler M
Efficient Detection of Hydrogen Bonds in Dynamic Regions of RNA by Sensitivity-Optimized NMR Pulse Sequences

(2013) Angew Chem Int Ed Engl. DOI: 10.1002/anie.201304391 [Pubmed]

Py H(CC)NN-COSY

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J(N-N) correlation in isotope-labeled RNA

pulse program and Bruker AvanceIII dataset]


Dallmann A, Simon B, Duszczyk MM, Kooshapur H, Pardi A, Bermel W, Sattler M
Efficient Detection of Hydrogen Bonds in Dynamic Regions of RNA by Sensitivity-Optimized NMR Pulse Sequences

(2013) Angew Chem Int Ed Engl. DOI: 10.1002/anie.201304391 [Pubmed]

DE HCCH-TOCSY

Download E/AE (t1 & t2) => 2x sensitivity enhanced


Sattler M, Schwendinger MG, Schleucher J, and Griesinger C
Novel strategies for sensitivity enhancement in heteronuclear multi-dimensional NMR experiments employing pulsed field gradients.
(1995) J Biomol NMR, 6:11-22 (1995)
[Pubmed] [Springer Link]

CDCA(NCO)CAHA Download E/AE, 2×13C CT periods with Ca/Cd and Ca selective pulses; for assignment of proline stretches
Bottomley MJ, Macias MJ, Liu Z, Sattler M.
A novel NMR experiment for the sequential assignment of proline residues and proline stretches in 13C/ 15N labeled proteins.
(1999) J. Biomol. NMR 13, 381-385.
[Pubmed]
Adenine HCCH-COSY Download Long-range HCCH correlation in adenine bases
Simon B, Zanier K, Sattler M
A TROSY relayed HCCH-COSY experiment for correlating adenine H2/H8 resonances in uniformly 13C-labeled RNA molecules

(2001) J Biomol NMR, 20:173-176
[Pubmed] [Springer Link]
2D w2-filtered NOESY noexfcng for D2O samples; double-filter for 13Cali, single filter for 15N and 13Carom
Sattler M., Schleucher J., and Griesinger C.
Heteronuclear multidimensional NMR experiments for the structure determination of proteins in solution employing pulsed field gradients.
(1999) Prog. NMR Spectrosc. 34, 93-158.